LOCUS pEX-A128-nCoV_al 3033 bp ds-DNA circular SYN 03-APR-2020 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3033) AUTHORS . TITLE Direct Submission JOURNAL Exported Friday, Apr 3, 2020 from SnapGene Viewer 3.1.2 to Vector NTI(R) format http://www.snapgene.com COMMENT VNTDATE|909792000| COMMENT VNTDBDATE|909878400| COMMENT VNTNAME|pEX-A128-nCoV_all| FEATURES Location/Qualifiers misc_feature 129..711 /label=all protein_bind 752..768 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(776..806) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 821..842 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(1130..1718) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(1889..2749) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" promoter complement(2750..2854) /gene="bla" /label=AmpR promoter ORIGIN 1 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta 61 actatgcggc atcagagcag attgtactga gagaaaggca attgggtacc gagctcgcgg 121 ccgcaagcat aatggacccc aaaatcagcg aaatgcaccc cgcattacgt ttggtggacc 181 ctcagattca actggcagta accagaatgg agaagaactg attacaaaca ttggccgcaa 241 attgcacaat ttgcccccag cgcttcagcg ttcttcggaa tgtcgcgcat tggcgttgca 301 actgagggag ccttgaatac accaaaagat cacattggca cccgcaatcc tgctaacaat 361 gctgcaatcg tgctacaact tcctcagtgt ttgcagattt ggacctgcga gcgggttctg 421 acctgaaggc tctgcgcgga cttgtggaga cagccgctca ccttggctat tgagtgaaat 481 ggtcatgtgt ggcggttcac tatatgttaa accaggtgga acctcatcag gagatgccac 541 aactgcttat gctaatagtg tttttaacat ttgtcaagct gaagagacag gtacgttaat 601 agttaatagc gtacttcttt ttcttgcttt cgtggtattc ttgctagtta cactagccat 661 ccttactgcg cttcgattgt gtgcgtactg ctgcaatatt gttaacgtga gacctgcttt 721 tgctcgcttg gatccgaatt caaaggtgaa attgttatcc gctcacaatt ccacacaaca 781 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat 841 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt 901 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct 961 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa 1021 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa 1081 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc 1141 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga 1201 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc 1261 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt 1321 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct 1381 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg 1441 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta 1501 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct 1561 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa 1621 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt 1681 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta 1741 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat 1801 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa 1861 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct 1921 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta 1981 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga ctcccacgct 2041 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg 2101 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa 2161 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt 2221 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta 2281 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca 2341 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta 2401 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct 2461 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg 2521 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac 2581 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact 2641 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa 2701 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt 2761 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat 2821 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg 2881 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc 2941 cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg 3001 agacggtcac agcttgtctg taagcggatg ccg //