LOCUS       pEX-A128-nCoV_WH        2695 bp ds-DNA     circular SYN 03-APR-2020
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Apr 3, 2020 from SnapGene Viewer 3.1.2 to Vector 
            NTI(R) format
            http://www.snapgene.com
COMMENT     VNTDATE|909792000|
COMMENT     VNTDBDATE|909878400|
COMMENT     VNTNAME|pEX-A128-nCoV_WHO|
FEATURES             Location/Qualifiers
     misc_feature    129..373
                     /label=WHO
     protein_bind    414..430
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(438..468)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    483..504
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(792..1380)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1551..2411)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
     promoter        complement(2412..2516)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta
       61 actatgcggc atcagagcag attgtactga gagaaaggca attgggtacc gagctcgcgg
      121 ccgcaagcct attgagtgaa atggtcatgt gtggcggttc actatatgtt aaaccaggtg
      181 gaacctcatc aggagatgcc acaactgctt atgctaatag tgtttttaac atttgtcaag
      241 ctgaagagac aggtacgtta atagttaata gcgtacttct ttttcttgct ttcgtggtat
      301 tcttgctagt tacactagcc atccttactg cgcttcgatt gtgtgcgtac tgctgcaata
      361 ttgttaacgt gagacctgct tttgctcgct tggatccgaa ttcaaaggtg aaattgttat
      421 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
      481 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
      541 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
      601 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
      661 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
      721 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
      781 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
      841 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
      901 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
      961 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     1021 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     1081 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     1141 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     1201 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     1261 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     1321 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     1381 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     1441 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     1501 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     1561 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     1621 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     1681 atgataccgc gactcccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     1741 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     1801 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     1861 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     1921 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     1981 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     2041 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     2101 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     2161 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     2221 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     2281 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     2341 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     2401 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     2461 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     2521 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
     2581 aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac
     2641 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccg
//