LOCUS       pEX-A128-nCoV_N3        2538 bp ds-DNA     circular SYN 03-APR-2020
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Apr 3, 2020 from SnapGene Viewer 3.1.2 to Vector 
            NTI(R) format
            http://www.snapgene.com
COMMENT     VNTDATE|909792000|
COMMENT     VNTDBDATE|909878400|
COMMENT     VNTNAME|pEX-A128-nCoV_N3|
FEATURES             Location/Qualifiers
     misc_feature    129..216
                     /label=N3
     protein_bind    257..273
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(281..311)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    326..347
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(635..1223)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1394..2254)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
     promoter        complement(2255..2359)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta
       61 actatgcggc atcagagcag attgtactga gagaaaggca attgggtacc gagctcgcgg
      121 ccgcaagcgc aactgaggga gccttgaata caccaaaaga tcacattggc acccgcaatc
      181 ctgctaacaa tgctgcaatc gtgctacaac ttcctcacct gcttttgctc gcttggatcc
      241 gaattcaaag gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
      301 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
      361 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
      421 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
      481 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
      541 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
      601 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
      661 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
      721 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
      781 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
      841 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
      901 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
      961 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     1021 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     1081 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     1141 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     1201 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     1261 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     1321 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     1381 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     1441 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     1501 taccatctgg ccccagtgct gcaatgatac cgcgactccc acgctcaccg gctccagatt
     1561 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     1621 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     1681 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     1741 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     1801 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     1861 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     1921 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     1981 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     2041 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     2101 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     2161 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     2221 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     2281 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     2341 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     2401 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc
     2461 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     2521 gtctgtaagc ggatgccg
//