LOCUS       pEX-A128-nCoV_N1        2536 bp ds-DNA     circular SYN 03-APR-2020
DEFINITION  synthetic circular DNA.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Apr 3, 2020 from SnapGene Viewer 3.1.2 to Vector 
            NTI(R) format
            http://www.snapgene.com
COMMENT     VNTDATE|909792000|
COMMENT     VNTDBDATE|909878400|
COMMENT     VNTNAME|pEX-A128-nCoV_N1|
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label=source
     misc_feature    129..214
                     /label=N1
     protein_bind    255..271
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(279..309)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    324..345
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(633..1221)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1392..2252)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
     promoter        complement(2253..2357)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta
       61 actatgcggc atcagagcag attgtactga gagaaaggca attgggtacc gagctcgcgg
      121 ccgcaagctg ataatggacc ccaaaatcag cgaaatgcac cccgcattac gtttggtgga
      181 ccctcagatt caactggcag taaccagaat ggagacctgc ttttgctcgc ttggatccga
      241 attcaaaggt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
      301 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
      361 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
      421 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
      481 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
      541 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
      601 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
      661 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
      721 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
      781 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
      841 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
      901 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
      961 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     1021 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     1081 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     1141 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     1201 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     1261 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     1321 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     1381 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     1441 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     1501 ccatctggcc ccagtgctgc aatgataccg cgactcccac gctcaccggc tccagattta
     1561 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     1621 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     1681 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     1741 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     1801 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     1861 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     1921 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     1981 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     2041 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     2101 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     2161 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     2221 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     2281 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     2341 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
     2401 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt
     2461 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
     2521 ctgtaagcgg atgccg
//