LOCUS pEX-A128-nCoV_CD 2788 bp ds-DNA circular SYN 03-APR-2020 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2788) AUTHORS . TITLE Direct Submission JOURNAL Exported Friday, Apr 3, 2020 from SnapGene Viewer 3.1.2 to Vector NTI(R) format http://www.snapgene.com COMMENT VNTDATE|909792000| COMMENT VNTDBDATE|909878400| COMMENT VNTNAME|pEX-A128-nCoV_CDC| FEATURES Location/Qualifiers misc_feature 129..466 /label=CDC protein_bind 507..523 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(531..561) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 576..597 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(885..1473) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(1644..2504) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" promoter complement(2505..2609) /gene="bla" /label=AmpR promoter ORIGIN 1 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta 61 actatgcggc atcagagcag attgtactga gagaaaggca attgggtacc gagctcgcgg 121 ccgcaagcat aatggacccc aaaatcagcg aaatgcaccc cgcattacgt ttggtggacc 181 ctcagattca actggcagta accagaatgg agaagaactg attacaaaca ttggccgcaa 241 attgcacaat ttgcccccag cgcttcagcg ttcttcggaa tgtcgcgcat tggcgttgca 301 actgagggag ccttgaatac accaaaagat cacattggca cccgcaatcc tgctaacaat 361 gctgcaatcg tgctacaact tcctcagtgt ttgcagattt ggacctgcga gcgggttctg 421 acctgaaggc tctgcgcgga cttgtggaga cagccgctca ccttggacct gcttttgctc 481 gcttggatcc gaattcaaag gtgaaattgt tatccgctca caattccaca caacatacga 541 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt 601 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga 661 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc 721 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg 781 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc 841 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc 901 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga 961 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc 1021 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat 1081 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg 1141 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc 1201 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga 1261 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact 1321 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt 1381 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag 1441 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg 1501 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa 1561 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata 1621 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg 1681 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata 1741 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgactccc acgctcaccg 1801 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct 1861 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt 1921 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc 1981 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga 2041 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt 2101 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc 2161 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa 2221 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca 2281 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca 2341 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct 2401 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc 2461 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa 2521 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt 2581 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc 2641 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt 2701 cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg 2761 gtcacagctt gtctgtaagc ggatgccg //